![]() As a result, the loss of temperature of the pre-heated collection cup may lead to cold temperature shock of the sperm cells, depending on the ambient temperature. Temperature shock is a possibility at the time of semen collection because the semen coming from the boar will be at body temperature, but the pre-heated collection cup may not be able to maintain its heated temperature because the boar generally takes 5 to 10 minutes to complete ejaculation. With current boar semen collection protocols, the semen is normally collected into a collection cup that has been heated to a temperature that closely matches the boar's body temperature (approximately 38☌ Flowers, 2017) in order to prevent temperature shock to the sperm cells. These data suggest that boar semen ejaculates collected into a collection cup and plastic collection bag containing 100 mLs of semen extender warmed to 38.5☌ will have greater percentages of motile and progressively motile sperm compared to boar sperm collected into a collection cup and plastic collection bag warmed to 38.5☌ containing no semen extender. No differences (P = 0.96) were present for normal sperm morphology in the treatment compared to the control (89.1 vs. 75.2%) and sperm progressive motility (64.1 vs. Statistical differences (P = 0.03) were found between the treatment and control for sperm motility (82.2 vs. There was no day x treatment effect (P > 0.05). Motility and morphology were evaluated on day 0 (day of collection) and day 6. Following collection and processing, the semen was extended to 37.5 × 10 6 sperm/mL and stored for 6 days post-collection in a semen cooler at 17☌. Every other collection, the boar's ejaculate was collected into a collection cup and plastic collection bag warmed to 38.5☌ containing either no semen extender (control) or 100 mLs of a commercially available long-term semen extender warmed to 38.5☌ (treatment). Sexually mature Berkshire x Duroc crossbred boars (n = 7) were semen collected once per week for four consecutive weeks. The purpose of this research was to determine if collecting boar semen directly into semen extender warmed to 38.5☌ would affect sperm quality post-collection. If the ambient temperature does not closely match the temperature of the warmed collection cup and semen at the time of collection then negative effects to the motility and morphology of the sperm cells may occur due to temperature shock. Semen used for artificial insemination (AI) in the swine industry is typically collected into a warmed semen collection cup containing an empty collection bag. ![]()
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